helicobacter pylori-induced genotoxic damage in human b lymphocytes
نویسندگان
چکیده
background helicobacter pylori (h. pylori) is recognized as the causative agent of peptic and duodenal ulcers, gastric adenocarcinoma, and low-grade mucosa-associated lymphoid tissue (malt) lymphoma . in the present study, we investigate the genotoxic damage of lysates of h. pylori in human b lymphocytes. materials and methodsâ human b lymphocytes were treated with 0, 10, 20, and 30 âµg/ l of total protein concentration of lysates obtained from h. pylori isolates from dyspeptic patients. direct h. pylori- induced dna damage was investigated by the in vitro cytokinesis-block cytokinesis- assay which detects chromosomal fragments and maldistributed whole chromosomes. resultsâ the total mean micronuclei number â (tmmn) observed per 1000 binucleus b cells significantly correlated with increasing protein concentration of h. pylori lysates ( 12.43 â± 1.91) when compared with the control (3.26 â± 0.48) . conclusion this study provides evidence of the direct effect of h. pylori in chromosomal breakage of human b lymphocytes, which might lead to the development of abnormal b cells. long-term infection by h. pylori has been implicated in epithelial cell damage as a result of continuous induction of the immune system by bacterial antigens. however, the results of this study propose that persistent h. pylori infection could also directly damage b lymphocyte dna from which gastric maltâ â lymphoma arises.
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گوارشجلد ۱۷، شماره ۴، صفحات ۲۷۴-۰
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